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| Registros recuperados: 76 | |
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| Fuhrmann, Marine; Petton, Bruno; Quillien, Virgile; Faury, Nicole; Morga, Benjamin; Pernet, Fabrice. |
| Mortality of young Pacific oysters Crassostrea gigas associated with the ostreid herpesvirus 1 (OsHV-1) is occurring worldwide. Here, we examined for the first time the effect of salinity on OsHV-1 transmission and disease-related mortality of C. gigas, as well as salinity-related effects on the pathogen itself. To obtain donors for OsHV-1 transmission, we transferred laboratory-raised oysters to an estuary during a disease outbreak and then back to the laboratory. Oysters that tested OsHV-1 positive were placed in seawater tanks (35‰, 21°C). Water from these tanks was used to infect naïve oysters in 2 experimental setups: (1) oysters acclimated or non-acclimated to a salinity of 10, 15, 25 and 35‰ and (2) oysters acclimated to a salinity of 25‰; the... |
| Tipo: Text |
Palavras-chave: Bivalve; Herpesviridae; Infection; Disease transmission; Risk analysis. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00350/46100/45781.pdf |
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| Muir, Anna; Nunes, Flavia; Dubois, Stanislas; Pernet, Fabrice. |
| Acclimation and adaptation, which are key to species survival in a changing climate, can be observed in terms of membrane lipid composition. Remodelling membrane lipids, via homeoviscous adaptation (HVA), counteracts membrane dysfunction due to temperature in poikilotherms. In order to assess the potential for acclimation and adaptation in the honeycomb worm, Sabellaria alveolata, a reef-building polychaete that supports high biodiversity, we carried out common-garden experiments using individuals from along its latitudinal range. Individuals were exposed to a stepwise temperature increase from 15 °C to 25 °C and membrane lipid composition assessed. Our results suggest that S. alveolata was able to acclimate to higher temperatures, as observed by a... |
| Tipo: Text |
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| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00354/46551/46361.pdf |
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| Gangnery, Aline; Normand, Julien; Duval, Cyrielle; Cugier, Philippe; Grangeré, Karine; Petton, Bruno; Petton, Sebastien; Orvain, Francis; Pernet, Fabrice. |
| Oyster diseases have major consequences on fisheries and aquaculture. In France, young Pacific oysters Crassostrea gigas are severely hit by the ostreid herpesvirus, whereas adults suffer mortalities presumably caused by pathogenic bacteria. Here we investigated the origin and spread of mortalities that affect both young and adult oysters, and we identified and compared their risk factors. Mortality was monitored in 2 age classes of oysters deployed in early spring at 39 sites spread over a 37 km2 surface area inside and outside of shellfish farms. Environmental data obtained from numerical modelling were used to investigate risk factors. Mortality of young oysters associated with ostreid herpesvirus occurred in the oyster farming area. Hydrodynamic... |
| Tipo: Text |
Palavras-chave: Aquaculture; Bivalve; Disease; Epidemiology and health; Ecological modelling; Hydrodynamic connectivity. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00588/69983/67896.pdf |
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| Pernet, Fabrice; Lugue, Klervi; Petton, Bruno. |
| Competition between organisms interfere in host and pathogen dynamics in ways that are difficult to predict. By one side, competitors can reduce the food supply and cause nutritional stress. Such stress could further modulate the susceptibility to infection by altering immune response or metabolic rate of the host. Alternatively, competitors may trap pathogens before they reach the focal host, and therefore reduce, enhance, or have no effect on infection according to the competitor's susceptibility to the infection. To better understand how competition influences host and pathogen interactions, we experimentally assessed the relative importance of competition for pathogens and resources on the severity of a viral disease infecting the Pacific oyster... |
| Tipo: Text |
Palavras-chave: Competition; Crassostrea gigas; Disease ecology; Filtration; Ostreid herpesvirus 1; Pathogen. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00692/80375/83493.pdf |
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| Lagarde, Franck; Roque D'Orbcastel, Emmanuelle; Perignon, Adeline; Mortreux, Serge; Fuhrmann, Marine; Le Gall, Patrik; Leurion, Axel; Chiantella, Claude; Bec, Beatrice; Roques, Cecile; Bonnet, Delphine; Cochet, Helene; Boj, Marie; Gervasoni, Erika; Miron, Gilles; Fiandrino, Annie; Pouvreau, Stephane; Pernet, Fabrice. |
| We bring new items in connection with the origins of the natural recruitment variability of the Pacific oyster Crassostrea gigas in Thau lagoon. A poor relationship between " number of larvae" and "number of spat" in 2012 shows a biological lock around oysterculture areas during the metamorphosis in spite of better success of recruitment outside shellfish farming zone. A comprehensive analysis of environmental factors effects and the development of prognosis tools are planned to assist oysterfarmers in rationalization of natural oyster recruitment around Mediterranean lagoon. |
| Tipo: Text |
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| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00165/27653/25822.pdf |
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| Gagne, Renee; Tremblay, Rejean; Pernet, Fabrice; Miner, Philippe; Samain, Jean-francois; Olivier, Frederic. |
| The main objective of this study was to evaluate the effect of the addition of Rhodomonas salina in the diet of Pecten maximus on growth, survival and metamorphosis success in relation to biochemical content. Food quality is an essential factor in the success of larval, post-larval and juvenile development. A diet rich in polyunsaturated fatty acids present an advantage for growth, survival and metamorphosis success. Larvae and post-larvae fed with the diet containing R. sauna showed a higher accumulation of arachidonic acid (20:4n-6) and generally a lower concentration of docosahexaenoic acid (22:6n-3). Addition of R. sauna in diet was related with higher level of eicosapentaenoic acid (20:5n-3) in larval stages, but lower level in post-larvae. Addition... |
| Tipo: Text |
Palavras-chave: Nutrition; Biochemical characteristics; Fatty acids; Sterol profiles. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00019/13045/10205.pdf |
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| Curd, Amelia; Pernet, Fabrice; Corporeau, Charlotte; Delisle, Lizenn; Firth, Louise B.; Nunes, Flavia; Dubois, Stanislas. |
| The honeycomb worm Sabellaria alveolata is capable of building extensive bioconstructions, including what are currently considered Europe’s largest biogenic reefs. The size and volume of these bioconstructions, however, vary greatly, such that not all habitats engineered by S. alveolata may be easily identified as reefs. Given that European environmental legislation protects marine habitats that are classified as “reefs”, it is important to identity a clear set of definition criteria. Furthermore, quantifiable and unequivocal criteria are also needed to evaluate the ecological (health) state of these reefs, in order to best monitor and protect them. Here we propose new terminology to describe the physical appearance of these bioconstructions and attempt to... |
| Tipo: Text |
Palavras-chave: Biochemical indicators; Health; Ecological status; Sabellaria alveolata; Biogcnic reef; Engineer species. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00464/57608/60175.pdf |
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| Degremont, Lionel; Maurouard, Elise; Nourry, Max; Seugnet, Jean-luc; Bedier, Edouard; Fleury, Elodie; Langlade, Aime; Pernet, Fabrice; Pouvreau, Stephane; Le Souchu, Pierrick; Normand, Julien; D'Amico, Florence; Rumebe, Myriam; Cantin, Christian; Barret, Jean; Le Gall, Patrik; Baud, Jean-pierre; Cochennec-laureau, Nathalie; Gervasoni, Erika; Pelissier, Pablo. |
| Ce rapport présente la comparaison des mortalités de deux lots de naissains de Crassostrea gigas dans plusieurs bassins conchylicoles et déterminer le gain de survie obtenu par la sélection pour des huîtres diploïdes |
| Tipo: Text |
Palavras-chave: Mortalités; Huître creuse; Crassostrea gigas; Sélection génétique; Naissain. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00065/17644/15180.pdf |
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| Corporeau, Charlotte; Tamayo, David; Pernet, Fabrice; Quere, Claudie; Madec, Stephanie. |
| Pacific oyster Crassostrea gigas were inoculated with OsHV-1 at low load (control) or high load (challenged) to better understand the pathogenesis of ostreid herpesvirus 1 (OsHV-1 μVar) and to determine which metabolic pathways might be affected during infection. Animals were sampled for proteomic analysis two days post-injection, at the same time as OsHV-1 initiated an intense replication phase in challenged oysters. Twenty-five abundant protein spots that showed a marked change in accumulated levels were identified using a two-dimensional electrophoresis (2-DE) proteomic approach. Overall, these proteins are involved in cytoskeleton organization, protein turnover, induction of stress signals, signalling pathways and energy metabolism. Challenged oysters... |
| Tipo: Text |
Palavras-chave: Marine bivalves; Crassostrea gigas; Herpesvirus; Metabolism; Warburg. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00199/31035/29439.pdf |
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| Petton, Bruno; Destoumieux Garzon, Delphine; Pernet, Fabrice; Toulza, Eve; De Lorgeril, Julien; Degremont, Lionel; Mitta, Guillaume. |
| The Pacific oyster (Crassostreae gigas) has been introduced from Asia to numerous countries around the world during the 20th century. C. gigas is the main oyster species farmed worldwide and represents more than 98% of oyster production. The severity of disease outbreaks that affect C. gigas, which primarily impact juvenile oysters, has increased dramatically since 2008. The most prevalent disease, Pacific oyster mortality syndrome (POMS), has become panzootic and represents a threat to the oyster industry. Recently, major steps towards understanding POMS have been achieved through integrative molecular approaches. These studies demonstrated that infection by Ostreid herpesvirus type 1 µVar (OsHV-1 µvar) is the first critical step in the infectious process... |
| Tipo: Text |
Palavras-chave: Pacific oyster mortality syndrome; Polymicrobial disease; Multifactorial disease; Crassostrea gigas; OsHV-1; Opportunistic bacterial pathogens. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00679/79158/81669.pdf |
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| Normand, Julien; Li, Ronghua; Quillien, Virgile; Nicolas, Jean-louis; Boudry, Pierre; Pernet, Fabrice; Huvet, Arnaud. |
| Pacific oyster Crassostrea gigas suffers from chronic or sporadic mortality outbreaks worldwide, resulting from infectious diseases and/or physiological disorders triggered by environmental factors. Since 2008, ostreid herpesvirus OsHV-1 μVar has been identified as the main agent responsible for mass mortality of juvenile oysters in Europe. Previous studies of genome-wide expression profiling have provided candidate genes that potentially contribute to genetically-based resistance to summer mortality. To assess their value in determining resistance to the juvenile mass mortality that has occurred in France since 2008, we analyzed the expression of 17 candidate genes in an experimental infection by OsHV-1 μVar, and in an in vivo field experiment. Individual... |
| Tipo: Text |
Palavras-chave: Molluscs; Gene expression profiling; Inhibitor of kappa B; Summer mortality; Ostreid herpesvirus. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00177/28823/27325.pdf |
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| Pernet, Fabrice; Lagarde, Franck; Cesmat, Ludovic; Jeannée, Nicolas; Barret, Jean; Le Gall, Patrik; Quere, Claudie; Fiandrino, Annie; Miguet, Marine; Boulet, Hélène; Keck, Nicolas; Roque D'Orbcastel, Emmanuelle. |
| Cette étude avait pour objectif principal de déterminer les facteurs explicatifs de la dynamique spatio-temporelle des surmortalités d’huîtres creuses dans la lagune de Thau. Les facteurs étudiés étaient : la pathologie (détection d’ADN appartenant à l’Ostreid herpesvirus 1 OsHV-1 et au groupe bactérien Vibrio splendidus), l’état énergétique des huîtres (triglycérides, sucres totaux et acides gras comme marqueurs trophiques), les pratiques d’élevage et l’hydrodynamique. Afin de répondre à ces objectifs, nous avons suivi la mortalité d’un lot d’huîtres sentinelles, âgé de moins d’un an, maintenu indemne de mortalité, dans lequel on ne détectait pas d’ADN appartenant à OsHV-1. Le lot a été déployé mi-mars 2011 sur 106 stations dans la lagune de Thau, où les... |
| Tipo: Text |
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| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00092/20355/18015.pdf |
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| Delisle, Lizenn; Pauletto, Marianna; Vidal-dupiol, Jeremie; Petton, Bruno; Bargelloni, Luca; Montagnani, Caroline; Pernet, Fabrice; Corporeau, Charlotte; Fleury, Elodie. |
| Of all environmental factors, seawater temperature plays a decisive role in triggering marine diseases. Like fever in vertebrates, high seawater temperature could modulate the host response to pathogens in ectothermic animals. In France, massive mortality of Pacific oysters, Crassostrea gigas, caused by the ostreid herpesvirus 1 (OsHV-1) is markedly reduced when temperatures exceed 24°C in the field. In the present study we assess how high temperature influences the host response to the pathogen by comparing transcriptomes (RNA sequencing) during the course of experimental infection at 21°C (reference) and 29°C. We show that high temperature induced host physiological processes that are unfavorable to the viral infection. Temperature influenced the... |
| Tipo: Text |
Palavras-chave: Anti-viral molecular pathway; Host-pathogen interaction; Marine disease; OsHV-1; Resistance; Temperature. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00656/76806/77974.pdf |
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| Pernet, Fabrice; Gachelin, Sonia; Stanisière, Jean-yves; Petton, Bruno; Fleury, Elodie; Mazurié, Joseph. |
| The intertidal zone is characterized by a sharp vertical gradient of environmental stress, which structures species distribution and their interactions. Few studies, however, have examined the influence of tidal height on host–pathogen interactions. Here, we investigated how the tidal height influence outbreak of the Ostreid herpesvirus type 1 (OsHV-1) affecting the Pacific oyster. A volunteer network composed of 20 oyster growers monitored the survival of 28 batches of oysters during an epizootic event in Southern Brittany, France. Oysters were spat from wild collection or hatchery production. The sampling sites were spread over a 150-km2 area with a tidal height ranging from 0.98 to 2.90 m. Concomitantly, we followed survival of oyster spats in relation... |
| Tipo: Text |
Palavras-chave: Aquaculture; Citizen science; Marine epidemiology and health; OsHV-1; Risk analysis. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00491/60271/63981.pdf |
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| Masseret, Estelle; Banack, Sandra; Boumediene, Farid; Abadie, Eric; Brient, Luc; Pernet, Fabrice; Juntas-morales, Raoul; Pageot, Nicolas; Metcalf, James; Cox, Paul; Camu, William. |
| Background Dietary exposure to the cyanotoxin BMAA is suspected to be the cause of amyotrophic lateral sclerosis in the Western Pacific Islands. In Europe and North America, this toxin has been identified in the marine environment of amyotrophic lateral sclerosis clusters but, to date, only few dietary exposures have been described. Objectives We aimed at identifying cluster(s) of amyotrophic lateral sclerosis in the Hérault district, a coastal district from Southern France, and to search, in the identified area(s), for the existence of a potential dietary source of BMAA. Methods A spatio-temporal cluster analysis was performed in the district, considering all incident amyotrophic lateral sclerosis cases identified from 1994 to 2009 by our expert... |
| Tipo: Text |
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| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00168/27941/26234.pdf |
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| Epelboin, Yanouk; Quere, Claudie; Pernet, Fabrice; Pichereau, Vianney; Corporeau, Charlotte. |
| Here, we assess the physiological effects induced by environmental concentrations of pesticides in Pacific oyster Crassostrea gigas. Oysters were exposed for 14 d to trace levels of metconazole (0.2 and 2 mu g/L), isoproturon (0.1 and 1 mu g/L), or both in a mixture (0.2 and 0.1 mu g/L, respectively). Exposure to trace levels of pesticides had no effect on the filtration rate, growth, and energy reserves of oysters. However, oysters exposed to metconazole and isoproturon showed an overactivation of the sensing-kinase AMP-activated protein kinase alpha (AMPK alpha), a key enzyme involved in energy metabolism and more particularly glycolysis. In the meantime, these exposed oysters showed a decrease in hexokinase and pyruvate kinase activities, whereas 2-DE... |
| Tipo: Text |
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| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00284/39490/44227.pdf |
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| Ubertini, Martin; Lagarde, Franck; Le Gall, Patrik; Mortreux, Serge; Pernet, Fabrice; Fiandrino, Annie; Pouvreau, Stephane; Roque D'Orbcastel, Emmanuelle. |
| The aquaculture of the oyster Crassostrea gigas is largely based on natural recruitment of the species on spat collectors along the french Atlantic coast, the remaining spat being provided by hatcheries and nurseries. Due to an increase in the climate/meteorological variability, during the last twenty years, the natural spat collection has become variable, from null to overabundant depending on the year. Since 2008, the oyster spat mortalities worsen the situation, reducing the available spat and increasing the cost of the latter. However, the temporal variability of the natural recruitment modalities in the Mediterranean do not allow the aquaculture industry to rely on native spats, making the understanding of natural recruitment in the Mediterranean a... |
| Tipo: Text |
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| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00248/35894/34422.pdf |
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| Theriault, I; Pernet, Fabrice. |
| This study examines the effects of varying dietary lipids on growth, survival, lipid composition and behaviour in lobster Homarus americanus (Milne Edwards, 1837) postlarvae. Lobsters were fed one of 3 diets during their early ontogeny: (1) Artemac, a widely used Artemia replacement; (2) a ternary mixture consisting of Artemac, frozen Artemia adults and Artemia flakes; or (3) a mixture of live A. salina nauplii and adults. We evaluated the olfactory capacity (response to predator odour plume) and cryptic behaviour (ability to hide during settlement) of lobster postlarvae as a function of these diets and the lipid and the fatty acid composition of lobsters. Growth and survival of lobster postlarvae were highest in those fed live Artemia, followed by the... |
| Tipo: Text |
Palavras-chave: Settlement behaviour; Nutrition; Lipid; Habitat selection; Fatty acid; Crustacean larvae. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-4651.pdf |
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| Registros recuperados: 76 | |
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